Regulatory

Part:BBa_K5477009:Design

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)


Lex6Op - six tandem copies of LexA operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our reporter module, the Lex6Op is positioned upstream of the promoter (pLEU2), meaning the LexA fused with either the wildtype or mutant ERα or ERRγ will bind to these operators that drive the expression of our reporter gene NanoLuc.


Source

E. coli>

===References===