Regulatory
Part:BBa_K5477009:Design
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
Lex6Op - six tandem copies of LexA operator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our reporter module, the Lex6Op is positioned upstream of the promoter (pLEU2), meaning the LexA fused with either the wildtype or mutant ERα or ERRγ will bind to these operators that drive the expression of our reporter gene NanoLuc.
Source
E. coli>
===References===